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Why the order we draw your tubes matters

The tubes are not empty, and what is in one can change the numbers in the next. The sequence is the only thing that prevents it.

By Suvani Jayawardhana··2 min read

If you have had blood taken for more than one test, you will have noticed that several tubes get filled from one needle, one after another, and that they have different coloured caps. The colours are not decoration and the order is not habit. It is worth thirty seconds of your attention, because it is the kind of care you only notice when it is missing.

The tubes are not empty

Each tube already contains something chosen for the tests it carries. The gold topped tube has a clot activator and a gel. The grey one has sodium fluoride, which stops the cells in the sample from eating the sugar you came to have measured. The lavender topped tube contains EDTA.

EDTA is what stops blood clotting so that a full blood count can be done at all, and it does that by binding up calcium. It is also a potassium salt.

What happens if the sequence is wrong

If a needle carries a trace of EDTA from one tube into the next, that next tube reads with falsely raised potassium and falsely lowered calcium.

Not wildly wrong. Plausibly wrong, which is far worse. A potassium high enough to be flagged critical means somebody telephoning a doctor about a heart risk the patient does not have. A calcium low enough to be flagged means somebody investigating a parathyroid gland that is working perfectly well. Both start with a result that looked entirely believable on the page.

The prevention is an order, not a machine

There is no clever instrument that fixes this. What fixes it is filling the tubes in a fixed clinical sequence, every single time: citrate, then serum, then heparin, then EDTA, then fluoride. The additive that would do the most damage downstream is drawn last.

The lavender tube is also never spun in the centrifuge. A full blood count needs the cells whole and in suspension, and a spun EDTA tube is not a result, it is a second needle for the patient. Handling instructions print on the label rather than depending on somebody remembering them at half past six in the morning.

The same applies at your door

None of this changes when we come to your home. The same tubes, filled in the same order, by somebody who does this all day, and brought back in a proper carrier rather than a bag on a seat. A home visit is a convenience for you. It is not a shortcut for us.

Why write this down

You cannot inspect a laboratory from the waiting room. You can see whether the floor is clean and whether somebody smiled at you, and neither has anything to do with whether your potassium is really your own.

What you can do is ask how a place handles this, and listen for whether the answer is specific. Ours is above, and you are welcome to ask us about any other part of it.

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